LungMosaic characteristics

 Our model uniquely represents multiple lung regions within a single system, unlike alveolospheres or bronchospheres, which are limited to the alveoli or bronchi, respectively. This heterogeneity enhances the model’s relevance for studying diseases where dynamic crosstalk between proximal and distal lung regions is critical, such as asthma, COPD, and viral infections.

 Unlike traditional models derived from adult stem cells, our organoids exhibit a tubular morphology that mirrors the lung's natural branching morphogenesis. This structural complexity enhances their physiological relevance and directly impacts their functional performance. 

 Derived from tracheal cells, our model enhances scalability by providing access to samples from multiple donors, while mitigating the high risk and cost associated with deep lung tissue biopsies required by existing commercial models. Alternatively, bronchial epithelial cells or immortalized cell lines can also be used. 

 Validated through transcriptomic and proteomic analysis, our models show greater resemblance to adult lung tissue than conventional systems, ensuring consistency and predictive power for drug development. 

 Our protocol enables the generation of advanced lung in vitro models from multiple donors, including healthy males and females, as well as patients with asthma, emphysema, and viral infections, across diverse ages and disease severities. This paves the way for applications in personalized medicine and patient-specific drug testing 

 Our models are designed to be cryopreserved and delivered in ready-to-use formats—available in both 12-well and 96-well plates—allowing seamless integration into research pipelines and accelerating experimental workflows. 

 By better replicating native lung physiology, our models increase the predictive accuracy of drug screening, offering a more reliable platform for preclinical testing, reducing downstream development failures, and associated costs.